High-Performance Liquid Chromatography (HPLC)
in ChemistryAbout this course
Introduction to HPLC : Principles and Applications
Are you curious about how High-Performance Liquid Chromatography (HPLC) works ? This analytical technique separates the compounds in a mixture using a pressurized liquid mobile phase flowing through a stationary phase contained within a column.
An HPLC instrument typically consists of four key components :
- A pump, ensuring the pressurized flow of the mobile phase.
- An autosampler, precisely injecting samples.
- A column compartment, where compound separation occurs.
- A detector, identifying and quantifying analytes.
The success of a separation depends on various parameters, such as the composition of the mobile and stationary phases, as well as temperature. Choosing the right stationary phase is crucial to differentiating analytes based on their respective affinities.
HPLC Applications
HPLC plays a vital role in numerous industries, including :
- Pharmaceuticals : impurity control and drug validation.
- Food and beverage : analysis of ingredients and additives.
- Environmental monitoring : detection of pollutants in water and soil.
By ensuring the quality and safety of everyday products, HPLC stands out as an essential technology in analytical chemistry.
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This whiteboard video provides a brief history of chromatography and explains the fundamentals of HPLC and UHPLC. It also describes the essential components, or modules, of a chromatography system.
Capillaries and fittings complete an HPLC system by connecting the various hardware components so that the mobile phase and sample mixture circulate continuously at high pressure.
A chromatographic data system (CDS) is a software package that controls all the components of an HPLC system, including operation, mobile phase mixing, pump flow, etc...
Prevent failed HPLC runs and protect your system with these essential tips. Learn best practices to optimize performance and extend the lifespan of your equipment.
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